Next generation sequencing

Next Generation Sequencing, or NGS, is a technology that enables massive parallel multiplexed analysis for billions of sequences simultaneously. It can be used to query the entire genome - known or unknown, or targeted at certain genomic region of interest such as cancer hot-spots. RNA molecules can also be converted to cDNA and therefore the entire transcriptome can be quantitatively analyzed using NGS. Because of its versatility, NGS is quickly becoming the most potent tool in genomics research.  

Our main workhorses are the Illumina line of next generation sequencers, from the desktop MiSeq to NovaSeq 6000, the sequencer with the highest capacity currently on the market. With these versatile, industry gold standard instruments, we can provide:

• Whole genome sequencing (WGS) for resequencing or de novo sequencing
• Whole exome sequencing (WES) for human or mouse
• Whole transcriptome analysis for mammalian, plants, and bacteria RNA
• Small RNA sequencing
• Methylation analysis – whole genome or reduced representation
• ChIP-Seq
• Microbiome shotgun whole genome sequencing
• 16S metagenomics sequencing

For projects requiring single molecule long reads, the PacBio Sequel II and Oxford Nanopore sequencers (Third Generation Sequencers) are available. 

Additionally, we provide one year, free-of-charge, sample storage and acquisition after project completion. After that, a nominal annual fee is applied to biobanking in our temperature-monitored ultra-low freezers.